bio gel p6 micro biospin columns (Bio-Rad)

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Bio-Rad bio gel p6 micro biospin columns
Bio Gel P6 Micro Biospin Columns, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 638 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/micro+biospin+p6+columns/Micro+Bio-Spin+P-6+Gel+Columns/10__1038_slash_s44383___025___00005___5-226-5-10
Average 96 stars, based on 638 article reviews

bio gel p6 micro biospin columns - by Bioz Stars, 2026-09

96/100 stars

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Western Blot:

Article Title: S-nitrosylation of NADPH oxidase regulates cell death in plant immunity.
Article Snippet: .. Donors were removed using Micro BioSpin P6 columns (BioRad) and the resulting proteins were subjected to the biotinswitch technique22 by western blot assay using anti-biotin antibody (New England Biolab). .. For in vivo assay, Arabidopsis plants were inoculated with Pst DC3000 (avrB) at 107 c.f.u. ml21 and an anti-Myc9 antibody (Sigma) was used.

Filtration:

Article Title: An RNA molecule that specifically inhibits G-protein-coupled receptor kinase 2 in vitro
Article Snippet: In brief, the incubation of the target protein with the RNA library was done in PBS (pH 7.4) and 3 mM MgCl 2 at 37°C for 30 min. GRK2 (1 μg/μL) was biotinylated with sulfo-NHS-LC biotin (Pierce) according to the manufacturer's protocol in the presence of 10 mM MgCl 2 and 100 μM ATP to avoid modification of the K220 residues within the catalytic core of GRK2. .. The nonreacted biotin-esters were removed from the protein solution by gel filtration using Micro Biospin P6 columns (BioRad). .. The biotinylated protein was subsequently coupled to 5 mg streptavidin beads (Dynal) and the resulting beads (267 μg per cycle) were directly used for the selection process.

Article Title: An RNA aptamer that induces transcription.
Article Snippet: The incubation of the target protein with the RNA library was made in phosphate-buffered saline (PBS) (pH 7.4) supplemented with 0.8 mg/ml bovine serum albumin and 3 mM MgCl2 at 37 C for 30 min. TetR (1 mg/ml) was biotinylated with a 5-fold molar excess of sulfo-NHS-LC biotin (Pierce) according to the manufacturer’s protocol. .. The nonreacted biotinesters were removed from the protein solution by gel filtration using Micro Biospin P6 columns (Bio-Rad). .. The biotinylated protein was subsequently coupled to 5 mg streptavidin beads (Invitrogen) and the resulting beads (267 mg/cycle) were directly used for the selection process.

Conjugation Assay:

Article Title: Methods for directed folding assembly or dimerization of proteins by templated assembly reactions
Article Snippet: To prepare for conjugation, 5 nmol of oligonucleotides modified at their 5′- or 3′-ends with disulfide moieties (IDT Corp.) were treated with 1 mM tris-carboxyethylphosphine (TCEP) for 12-16 hours in 50 μl PBS to reduce the disulfides and produce free thiol groups. .. Following this, the reduced oligonucleotides were desalted on Micro-Biospin P6 columns (Bio-Rad) into 10 mM Tris pH 7.4, and then used immediately for conjugation. ..

Purification:

Article Title: Ordered assembly of the V(D)J synaptic complex ensures accurate recombination
Article Snippet: .. Annealed complexes were purified through micro-BioSpin P6 columns (Bio-Rad) in 10 mM Tris pH 7.0 buffer. .. For radiolabeled substrates, one oligonucleotide in each complex (DAR39, DG61 or DAR42) was phosphorylated using T4 polynucleotide kinase (New England Biolabs) and [γ- 32 P]ATP (Dupont-NEN), then heated to 80°C for 20 min prior to annealing.

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